Continuing our new series of chemical recipes, we bring you week 29 – Biuret Reagent (C2H5N3O2)
Remember that when preparing any of our recipes, you should:
- Always wear appropriate PPE including eye protection and gloves.
- Always add acid to water (never water to acid).
- Use a fume cupboard.
- Always carry out a risk assessment when using any chemicals.
- Follow all recommended safety procedures and adhere to the label instructions, hazard warnings and local legislations.
SOLUTION A: Biuret’s Reagent, Qualitative, Sodium Hydroxide Solution
0.4M Sodium Hydroxide (CORROSIVE) – 16g Sodium Hydroxide made up to 1000ml with (distilled/ deionised) water.
SOLUTION B: Biuret’s Reagent, Qualitative, Sodium Hydroxide Solution
0.01M Copper (II) Sulphate (VI) – 2.5g Copper (II) Sulphate (IV) made up to 1000ml with (distilled/ deionised) water.
For the test, mix SOLUTION A with the test solution in a ratio of 1:1. Add SOLUTION B one drop at
a time, shaking well after each addition. A purple or pink colour shows the presence of protein.
Download the PDF recipe
Scan the QR codes for classroom experiments to follow with your biuret reagent.