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Recipe of the Week – Week 29: Biuret Reagent

Continuing our new series of chemical recipes, we bring you week 29 – Biuret Reagent (C2H5N3O2)

Remember that when preparing any of our recipes, you should:

  • Always wear appropriate PPE including eye protection and gloves.
  • Always add acid to water (never water to acid).
  • Use a fume cupboard.
  • Always carry out a risk assessment when using any chemicals.
  • Follow all recommended safety procedures and adhere to the label instructions, hazard warnings and local legislations.

Recipe(s):

SOLUTION A: Biuret’s Reagent, Qualitative, Sodium Hydroxide Solution
0.4M Sodium Hydroxide (CORROSIVE) – 16g Sodium Hydroxide made up to 1000ml with (distilled/ deionised) water.

SOLUTION B: Biuret’s Reagent, Qualitative, Sodium Hydroxide Solution
0.01M Copper (II) Sulphate (VI) – 2.5g Copper (II) Sulphate (IV) made up to 1000ml with (distilled/ deionised) water.
For the test, mix SOLUTION A with the test solution in a ratio of 1:1. Add SOLUTION B one drop at
a time, shaking well after each addition. A purple or pink colour shows the presence of protein.

Download the PDF recipe

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Scan the QR codes for classroom experiments to follow with your biuret reagent.