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In this experiment, students will explore the biological process of bacterial transformation using E. coli and plasmid DNA. At the end of the activity, students will have experience observing and analyzing acquired traits (ampicillin resistance and fluorescence) as exhibited by transformed bacterial cells.
• Explore genetic engineering and the central dogma with this elegant experiment
• Transform E.coli with pFluoroGreen™ - a plasmid containing genes for ampicillin resistance and the green fluorescent protein
• Select for transformed cells using LB-ampicillin plates and calculate transformation efficiency
• Expose transformed cells to IPTG to demonstrate differential gene expression
• New transformation protocol has high transformation efficiency = great results!
